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Strand P, Palmer RM, Wilson RF. The presence of specific bacteria in subgingival plaque has been used as an indicator of active periodontal disease. The technique of subgingival sampling may conjecturally influence the identification and enumeration of microorganisms reported. In this study, paper point sampling and scaler sampling are compared. Methods: Samples were taken from the following intraoral sites in 35 patients with untreated chronic periodontitis before and 1.5, 3, and 6 months after non-surgical periodontal therapy: supra- and subgingival plaque from the deepest pockets in each sextant; pooled supra- and subgingival plaque from another six randomly selected, less affected teeth; mucosal swab samples from the tongue, tonsils, throat, and buccal mucosa; and stimulated and unstimulated saliva. Microbial species were A subgingival plaque sample was then obtained using a Gracey curette machined to a blade width of 0.5 mm to facilitate access to the subgingal area. The subgingival plaque from each site was collected by placing the curette at the apical extent of the gingival crevice and drawing it coronally with slight pressure against the tooth surface.
Subgingival plaque sampling and clinical recording (at baseline) and scaling and root planing was performed. Two weeks later the selected periodontal sites were submitted to one of the following treatments: Irrigation with a hydroalcoholic solution of propolis extract twice a week for 2 weeks (group A); irrigation with a placebo twice a week for 2 weeks (group B); or no additional treatment (group C). group Sample type Sampling method Metagenomic analysis Main Findings Authors’ Conclusion 1 Pre–post interventional study 31 enrolled 25 completed SRP NA Saliva and subgingival plaque Samples collected at baseline, and 2 weeks, 6 weeks, and 12 weeks after intervention: 1. Chewing-stimulated saliva sample collected from 8 a.m. to 3 p.m. 2. 1.
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1987 Sep;2(3):142-4. Sampling of subgingival plaque: a comparison of two methods using darkfield microscopy. Strand P, Palmer RM, Wilson RF. The presence of specific bacteria in subgingival plaque has been used as an indicator of active periodontal disease.
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Thus, at numer- ous time points, plaque samples Every individual was examined clinically and microbiologically using the CPITN index and collecting subgingival plaque samples. Each participant received a Periodontal parameters were recorded and deoxyribonucleic acid was extracted from the subgingival plaque and coronary atherosclerotic plaque samples of the The subgingival plaque samples will be analyzed to define the microbiotic profiles of the patients and the CGF determined to define their inflammatory The amount of stannous fluoride in GCF and subgingival plaque samples will be analyzed.. Registret för kliniska prövningar. ICH GCP. H2S production was measured with the bismuth method and subgingival plaque samples were analyzed for the presence of 20 bacterial species with the Subgingival plaque samples are also collected for microbiological analysis of the microbiota with focus on hydrogen sulfide producing bacteria. Samples are Subgingival plaque samples (SPS) and stimulated saliva samples (SSS) were periodontal outcome, periodontitis, saliva sample, subgingival plaque sample METHODS: Subgingival bacterial plaque was collected from women > or =6 The odds ratio of having P. aeruginosa at levels > or =1 x 10(5) in the sample and Fig.4. PCR analyses of subgingival plaque samples from subjects with and without bad breath. Begränsat utrymme gör provtagning den sunda subgingival sulcus av five putative periopathogenic bacteria in subgingival plaque samples.
Renvert S(1), Wikström M, Helmersson M, Dahlén G, Claffey N. Author information: (1)School of Dental Hygiene, Public Dental Service, Kristianstad, Sweden.
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Bacterial diversity in human subgingival plaque. national sample survey population. Reproducibility of subgingival bacterial samples from patients with Effect of oxybenzone on PGE2-production in vitro and on plaque and gingivitis in vivo.
Sampling of subgingival plaque: a comparison of two methods using darkfield microscopy. Strand P, Palmer RM, Wilson RF.
METHODS: In 50 patients with periodontitis, subgingival plaque was sampled from the deepest pocket of each quadrant by using paper points and by gaining saliva with saline mouthrinse. Analysis was performed using a commercially available polymerase chain reaction test for 11 periodontal pathogens. A Study to Evaluate Sampling Methods for Subgingival Plaque The safety and scientific validity of this study is the responsibility of the study sponsor and investigators.
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Klinisk prövning på Periodontitis: subgingival plaque sample, CGF
Significantly lower mean proportions of spirochetes and motile rods and higher proportions of non‐motile organisms were obtained with subgingival washing compared with curette sampling. The presence of specific bacteria in subgingival plaque has been used as an indicator of active periodontal disease. The technique of subgingival sampling may conjecturally influence the identification and enumeration of microorganisms reported. In this study, paper point sampling and scaler sampling are compared. Have minimum 6 sampling sites with bleeding and pocket depth ≥2mm but not deeper than 4mm. Exclusion Criteria: Have had a dental prophylaxis within 2 weeks of plaque sampling visits; Have taken antibiotics or used anti-gingivitis / anti-bacterial oral care products such as chlorhexidine or Listerine within 2 weeks of plaque sampling visits; Subgingival plaque samples within the deepest pocket of each tooth were taken and analysed by real-time polymerase chain reaction (PCR) for Actinobacillus actinomycetemcomitans (AA), Porphyromonas 1994-08-01 · A subgingival plaque sample was then obtained using a Gracey curette machined to a blade width of 0.5 mm to facilitate access to the subgingal area.
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However, the collection of saliva is simple and contains pathogens of all intraoral surfaces. The aim of this study is to investigate whether a sampling strategy with mouthrinse (mouthrinse sample [MSP]; test) leads to results comparable with standard sampling method the subgingival microbiota, pathogens, and plaque [12]. In the present study, a gingival retraction cord (a specific dentistry tool) was used to sampling GCF and plaque: this is a novel sampling method that is potentially more reproducible and less technique-sensitive than the paper point sampling method. Curette sampling and a subgingival washing technique were compared within and between paired sites on two separate occasions in 24 patients using darkfield microscopy. Significantly lower mean proportions of spirochetes and motile rods and higher proportions of non-motile organisms were obtained with subgingival washing compared with curette sampling.